期刊
FOOD & FUNCTION
卷 5, 期 1, 页码 167-175出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3fo60378d
关键词
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资金
- National Natural Science Foundation of China [81072570]
- National Scientific and Technological Major Project for Significant New Drugs Creation [2012ZX09502001-004]
- Training Program Foundation for the 333 High Level Talents of Jiangsu Province
- Project Program of State Key Laboratory of Natural Medicines, China Pharmaceutical University [JKGP201103]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20100096110004]
- Natural Science Foundation of Jiangsu Province of China [BK2011621]
- Fundamental Research Funds for the Central Universities [JKPZ2013014]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
An acidic polysaccharide (SGP-2), with a molecular weight of 1880 kDa, was purified from the defatted whole-plant of Sarcandra glabra (Thunb.) Nakai. SGP-2 is mainly composed of glucose, galactose, mannose, arabinose and galacturonic acid in a molar ratio of 12.19 : 8.68 : 6.03 : 1.00 : 15.24. The primary structure analysis reveals that SGP-2 consists of 1,4-linked alpha-D-galacturonic acid, methyl-esterified 1,4-linked a-D-galacturonic acid, 1,5-linked alpha-I-arabinose, 1,4-linked alpha-D-mannose, 1,6-linked beta-D-glucose and 1,3-linked beta-D-galactose with branch chains of 1,4,6-linked beta-D-glucose, 1,3,6-linked alpha-D-mannose and 1,4,6-linked alpha-D-galactose. The results of a cell viability assay and colony formation assay indicate that SGP-2 has a potent anti-proliferation activity on human osteosarcoma MG-63 cells. SGP-2 increases the proportion of apoptotic cells and activates caspase-3. In addition, the anti-proliferation effect induced by SGP-2 is blocked by the pan-caspase inhibitor. Moreover, SGP-2 inhibits the migratory capacity of MG-63 cells accompanied with the inhibition of receptor for advanced glycation end-products (RAGE) and nuclear factor-kappa B (NF-kappa B). Taken together, these results suggest that SGP-2 has anti-cancer potential in the treatment of human osteosarcoma.
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