期刊
CROP & PASTURE SCIENCE
卷 62, 期 9, 页码 765-775出版社
CSIRO PUBLISHING
DOI: 10.1071/CP11046
关键词
breeding; diversity; MALDI-TOF; phylogeny; protein profiling
Narrow-leafed lupin (NLL) is one of the major legume crops in Australian farming systems which is largely used as animal feed. Several modern cultivars have been developed through breeding making NLL feasible for use as human food. Significant health benefits have been recognised for NLL. The current study characterised protein polymorphism among 25 Australian cultivars through mass spectrometry (MALDI-TOF) with the aim of developing molecular breeding strategies to improve protein quality and content. A total of 364 seed protein mass peaks were clearly identified by MALDI-TOF and 50 protein mass peaks were cultivar specific. In addition, 9 protein mass peaks were found present in all cultivars and 61 protein mass peaks present in 2-3 cultivars only. Phylogenic analysis based on the protein profile categorised the cultivars into 2 major groups, which are broadly supported by pedigree information. The low proportion (2.4%) of common protein mass peaks among the cultivars suggested a high level of diversity in seed protein of NLL.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据