期刊
CONSERVATION GENETICS RESOURCES
卷 7, 期 1, 页码 57-59出版社
SPRINGER
DOI: 10.1007/s12686-014-0359-5
关键词
Noninvasive sampling; Species determination; Mammalia monitoring; Wildlife forensics
We described a technique for detecting mammal species, based on the analysis of a control region fragment of mitochondrial DNA by establishing taxonomic identity from non-invasive samples. We detected a polymorphic fragment that varies in sequence and length within different mammalian species but maintains its identity among individuals of the same species. We amplified a single fragment in all the mammalian species tested from tissue samples and identified feces samples at species level. The use of a unique set of primers to assess the presence of different mammal species with non-invasive sampling allowed us to differentiate sequences from more than one species per environmental sample. Thus, it constitutes a powerful molecular tool for inventory and description of the mammal diversity distribution in natural areas.
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