Isolation and characterization of 14 tetranucleotide microsatellite DNA markers in the elongate loach Leptobotia elongata (Cypriniformes: Cobitidae) using 454 sequencing technology

Anthropogenic factors have contributed to a severe decline of Leptobotia elongata. Conservation information of L. elongata will be required. Here, 14 tetranucleotide microsatellite DNA markers were developed from L. elongata through high-throughput (454) sequencing data. Twenty-four individuals of the species were genotyped using these markers. All of the loci displayed a high level of polymorphism with the number of alleles per locus ranged from 7 to 23, polymorphism information content ranged from 0.650 to 0.943. Observed and expected heterozygosities ranged from 0.609 to 1.000 and from 0.699 to 0.967, respectively. These markers provide an excellent toolkit to study population genetic structure and would benefit the conservation of this species.