Development and characterization of 22 microsatellite loci for the ringed salamander (Ambystoma annulatum) using paired-end Illumina shotgun sequencing

We isolated and screened 150 microsatellite loci from the ringed salamander, Ambystoma annulatum, an ambystomatid salamander endemic to the Interior Highlands. Twenty-two polymorphic loci were identified and pooled into two multiplex reactions. These loci will be valuable tools for assessing population genetic structure and connectivity across the landscape, and informing management of this species.