期刊
CONSERVATION GENETICS RESOURCES
卷 3, 期 3, 页码 589-592出版社
SPRINGER
DOI: 10.1007/s12686-011-9411-x
关键词
Conservation genomics; Microsatellite; Vipera ursinii; 454 sequencing
The Next Generation Sequencing (pyrosequencing) technique allows rapid, low-cost development of microsatellite markers. We have used this technology to develop 14 polymorphic loci for the endangered meadow viper (Vipera ursinii). Based on 37,000 reads, we developed primers for 66 microsatellite loci and found that 14 were polymorphic. The number of alleles per locus varies from 1 to 12 (for 30 individuals tested). At a cost of about 1/3 that of a normal microsatellite development, we were able to define enough microsatellite markers to conduct population genetic studies on a non-model species.
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