期刊
CONSERVATION GENETICS RESOURCES
卷 2, 期 1, 页码 127-129出版社
SPRINGER
DOI: 10.1007/s12686-010-9184-7
关键词
Intron; Nuclear loci; Primers; Testudines
资金
- National Science Foundation [DEB-0817042]
- California Department of Fish and Game [HBSDF15]
- UC Davis Agricultural Experiment Station
- Direct For Biological Sciences [1239961] Funding Source: National Science Foundation
- Division Of Environmental Biology [1239961] Funding Source: National Science Foundation
- Division Of Environmental Biology
- Direct For Biological Sciences [0817042] Funding Source: National Science Foundation
We used PCR amplifications to assess 120 previously described nuclear markers for phylogeographic and phylogenetic analysis in turtles. Twenty-seven of 120 markers amplified a single PCR product for both the western pond turtle (Emys marmorata) and the West African mud turtle (Pelusios castaneus), 71 amplified a single product in either E. marmorata or P. castaneus, and a subset of eight markers amplified single products across a test panel of 11 additional turtle species representing a broad sample of turtle diversity. Our ongoing research shows that nuclear markers developed for birds, squamates, and other vertebrate taxa can be useful for analyses of turtles, suggesting that many primers are transferable across large phylogenetic distances.
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