期刊
CLONING AND STEM CELLS
卷 10, 期 3, 页码 391-402出版社
MARY ANN LIEBERT INC
DOI: 10.1089/clo.2008.0010
关键词
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资金
- Lincy Foundation
- Gail and Richard Siegal Foundation
- Discovery Eye Foundation
- CHOC Foundation
- Guilds, and Padrinos
- Larry Hoag Foundation
- 2nd ONCE International Award for New Technologies for the Blind
- the Crown Princess Margareta's Committee for the Blind
- the Swedish Association of the Visually Impaired
- the Swedish Science Council (Medicine)
- the Minda de Gunzburg Research Center for Retinal Transplantation
- NIH [ROIRR13438, U42RR18877, NS044060, EY09595]
Work in rodents has demonstrated that progenitor transplantation can achieve limited photoreceptor replacement in the mammalian retina; however, replication of these findings on a clinically relevant scale requires a large animal model. To evaluate the ability of porcine retinal progenitor cells to survival as allografts and integrate into the host retinal architecture, we isolated donor cells from fetal green fluorescent protein (GFP)transgenic pigs. Cultures were propagated from the brain, retina, and corneo-scleral limbus. GFP expression rapidly increased with time in culture, although lower in conjunction with photoreceptor markers and filial fibrillary acid protein (GFAP), thus suggesting downregulation of GFP during differentiation. Following transplantation, GFP expression allowed histological visualization of integrated cells and extension of fine processes to adjacent plexiform layers. GFP expression in subretinal grafts was high in cells expressing vimentin and lower in cells expressing photoreceptor markers, again consistent with possible downregulation during differentiation. Cells survived transplantation to the injured retina of allorecipients at all time points examined (up to 10 weeks) in the absence of exogenous immune suppression without indications of rejection. These findings demonstrate the feasibility of allogeneic progenitor transplantation in a large mammal and the utility of the pig in ocular regeneration studies.
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