期刊
CYTOSKELETON
卷 67, 期 1, 页码 13-22出版社
WILEY
DOI: 10.1002/cm.20412
关键词
cytokinesis; cytokinetic apparatus; isolation and partial purification; actomyosin ring; Saccharomyces cerevisiae
类别
资金
- Swiss National Science Foundation (SNF)
- NIH [GM42759]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R37GM042759, R01GM042759] Funding Source: NIH RePORTER
Cytokinesis is the process by which a cell physically divides in two at the conclusion of a cell cycle. In animal and fungal cells, this process is mediated by a conserved set of proteins including actin, type II myosin, IQGAP proteins, F-BAR proteins, and the septins. To facilitate biochemical and ultrastructural analysis of cytokinesis, we have isolated and partially purified the Saccharomyces cerevisiae cytokinetic apparatus. The isolated apparatus contains all components of the actomyosin ring for which we tested actin, myosin heavy and light chain, and IQGAP as well as septins and the cytokinetic F-BAR protein, Hoflp. We also present evidence indicating that the actomyosin rings associated with isolated cytokinetic apparati may be contractile in vitro, and show preliminary electron microscopic imaging of the cytokinetic apparatus. This first successful isolation of the cytokinetic apparatus from a genetically tractable organism promises to make possible a deeper understanding of cytokinesis. (C) 2009 Wiley-Liss, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据