期刊
CELL ADHESION & MIGRATION
卷 8, 期 5, 页码 468-477出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/cam.29152
关键词
cell polarization; cell migration; fluorescence correlation spectroscopy; calibrated imaging; FRAP; stoichiometry
类别
Systems biology has recently achieved significant success in the understanding of complex interconnected phenomena such as cell polarity and migration. In this context, the definition of systems biology has come to encompass the integration of quantitative measurements with sophisticated modeling approaches. This article will review recent progress in live cell imaging technologies that have expanded the possibilities of quantitative in vivo measurements, particularly in regards to molecule counting and quantitative measurements of protein concentration and dynamics. These methods have gained and continue to gain popularity with the biological community. In general, we will discuss three broad categories: protein interactions, protein quantitation, and protein dynamics.
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