期刊
CANCER DISCOVERY
卷 8, 期 11, 页码 1422-1437出版社
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/2159-8290.CD-18-0385
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资金
- Fred Hutchinson Histopathology and small-animal imaging Shared Resources
- Research Histology Core and the Flow Cytometry Core at the UVA Medical Center
- NIH [R01CA200547, R01CA194461, R03CA215777, T32CA009657, P30CA015704, P30CA044579]
- American Cancer Society [RSG-15-066-01-TBG]
- David R. Jones Fund at University of Virginia
- International Association for the Study of Lung Cancer
- NATIONAL CANCER INSTITUTE [P30CA015704, R03CA215777, T32CA009657, R01CA200547, P30CA044579, R01CA194461] Funding Source: NIH RePORTER
CREBBP, encoding an acetyltransferase, is among the most frequently mutated genes in small cell lung cancer (SCLC), a deadly neuroendocrine tumor type. We report acceleration of SCLC upon Crebbp inactivation in an autochthonous mouse model. Extending these observations beyond the lung, broad Crebbp deletion in mouse neuroendocrine cells cooperated with Rb1/Trp53 loss to promote neuroendocrine thyroid and pituitary carcinomas. Gene expression analyses showed that Crebbp loss results in reduced expression of tight junction and cell adhesion genes, including Cdh1, across neuroendocrine tumor types, whereas suppression of Cdh1 promoted transformation in SCLC. CDH1 and other adhesion genes exhibited reduced histone acetylation with Crebbp inactivation. Treatment with the histone deacetylase (HDAC) inhibitor Pracinostat increased histone acetylation and restored CDH1 expression. In addition, a subset of Rb1/Trp53/Crebbp-deficient SCLC exhibited exceptional responses to Pracinostat in vivo. Thus, CREBBP acts as a potent tumor suppressor in SCLC, and inactivation of CREBBP enhances responses to a targeted therapy. SIGNIFICANCE: Our findings demonstrate that CREBBP loss in SCLC reduces histone acetylation and transcription of cellular adhesion genes, while driving tumorigenesis. These effects can be partially restored by HDAC inhibition, which exhibited enhanced effectiveness in Crebbp-deleted tumors. These data provide a rationale for selectively treating CREBBP-mutant SCLC with HDAC inhibitors. (c) 2018 AACR.
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