期刊
BIOMEDICAL OPTICS EXPRESS
卷 5, 期 10, 页码 3427-3433出版社
OPTICAL SOC AMER
DOI: 10.1364/BOE.5.003427
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资金
- National Institutes of Health (NIH) [R01EB014873]
- National Science Foundation of China [61475103]
- Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University (SZU) [GD201401]
- Natural Science Foundation of SZU
We report quantitative measurements of two-, three-, and four-photon excitation action cross sections of several commonly used fluorophores and fluorescent proteins at three different excitation wavelengths of 800 nm, 1300 nm, and 1680 nm. The measured cross section values are consistent with simple quantum mechanic estimations. These values indicate that the optimum repetition rate for deep tissue 3-photon microscopy is approximately 1 to 2 MHz. We further demonstrate that it is feasible to perform 4-photon fluorescence microscopy of GFP labeled microglia in mouse brain in vivo at 1700 nm. 4-photon excitation increases the accessibility of fluorophores at the long wavelength spectral window of 1700 nm. (C)2014 Optical Society of America
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