期刊
BIOMEDICAL OPTICS EXPRESS
卷 5, 期 6, 页码 1822-1838出版社
OPTICAL SOC AMER
DOI: 10.1364/BOE.5.001822
关键词
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资金
- National Institutes of Health [RO1 EB002583]
- Human Frontier Science Program (HFSP)
Image simulation remains under-exploited for the most widely used biological phase microscopy methods, because of difficulties in simulating partially coherent illumination. We describe an open-source toolbox, microlith (https://code.google.com/p/microlith), which accurately predicts three-dimensional images of a thin specimen observed with any partially coherent imaging system, as well as images of coherently illuminated and self-luminous incoherent specimens. Its accuracy is demonstrated by comparing simulated and experimental bright-field and dark-field images of well-characterized amplitude and phase targets, respectively. The comparison provides new insights about the sensitivity of the dark-field microscope to mass distributions in isolated or periodic specimens at the length-scale of 10nm. Based on predictions using microlith, we propose a novel approach for detecting nanoscale structural changes in a beating axoneme using a dark-field microscope. (C) 2014 Optical Society of America
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