期刊
BIOMEDICAL OPTICS EXPRESS
卷 4, 期 2, 页码 307-321出版社
OPTICAL SOC AMER
DOI: 10.1364/BOE.4.000307
关键词
-
资金
- NIH [R01-NS057476, P50-NS010828, P01-NS055104, R01-EB000790]
- AHA [11SDG7600037]
Minimally invasive, specific measurement of cellular energy metabolism is crucial for understanding cerebral pathophysiology. Here, we present high-resolution, in vivo observations of autofluorescence lifetime as a biomarker of cerebral energy metabolism in exposed rat cortices. We describe a customized two-photon imaging system with time correlated single photon counting detection and specialized software for modeling multiple-component fits of fluorescence decay and monitoring their transient behaviors. In vivo cerebral NADH fluorescence suggests the presence of four distinct components, which respond differently to brief periods of anoxia and likely indicate different enzymatic formulations. Individual components show potential as indicators of specific molecular pathways involved in oxidative metabolism. (c) 2013 Optical Society of America
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据