期刊
BIOMEDICAL OPTICS EXPRESS
卷 1, 期 2, 页码 729-735出版社
OPTICAL SOC AMER
DOI: 10.1364/BOE.1.000729
关键词
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资金
- EU [IST-2005-034562]
We present a simple approach in electrophoretic DNA separation and fluorescent monitoring that allows to identify the insertion or deletion of base-pairs in DNA probe molecules from genetic samples, and to perform intrinsic calibration/referencing for highly accurate DNA analysis. The principle is based on dual-point, dual-wavelength laser-induced fluorescence excitation using one or two excitation windows at the intersection of integrated waveguides and microfluidic channels in an optofluidic chip and a single, color-blind photodetector, resulting in a limit of detection of similar to 200 pM for single-end-labeled DNA molecules. The approach using a single excitation window is demonstrated experimentally, while the option exploiting two excitation windows is proposed theoretically. (C) 2010 Optical Society of America
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