期刊
ARABIAN JOURNAL OF CHEMISTRY
卷 7, 期 5, 页码 811-816出版社
ELSEVIER
DOI: 10.1016/j.arabjc.2012.07.010
关键词
Phenylephrine; Paracetamol; Caffeine; Chlorpheniramine; Simultaneous estimation; RP-HPLC-DAD; Tablet dosage
A simple, specific and accurate isocratic RP-HPLC-DAD method was developed for the simultaneous determination of phenylephrine, paracetamol, caffeine and chlorpheniramine in bulk and tablet dosage form. The four contents are present in variable concentrations and have variable chromatographic behavior making the process of analysis very difficult. For present studies a reversed-phase C-18 column (150 mm x 4.5 mm i.d., particle size 5 mu m) with mobile phase consisting of acetonitrile, methanol and 10 Mm phosphate buffer 16: 22: 62 (v/v) (pH of buffer 2.5 +/- 0.02, adjusted with ortho phosphoric acid) was used. The flow rate was 1.0 ml/min and eluents were monitored at 280 nm. The mean retention times of phenylephrine, paracetamol, caffeine and chlorpheniramine were found to be 1.8, 3.1, 5.2 and 10.9 min, respectively. The method was validated in terms of linearity, range, specificity, accuracy, precision and robustness. The proposed method was successfully applied to the estimation of phenylephrine, paracetamol, caffeine and chlorpheniramine in combined tablet dosage form. (C) 2012 Production and hosting by Elsevier B. V. on behalf of King Saud University.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据