期刊
ADVANCED ENERGY MATERIALS
卷 3, 期 7, 页码 881-893出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/aenm.201201014
关键词
dextran-wrapped nanoceria; cerium oxide (CeO2) nanoparticle; single-walled carbon nanotube (SWCNT); fullerenol (polyhydroxylated fullerene; buckeyball; C60); 2; 7-dichlorodihydrofluorescein diacetate (H2DCF-DA); 2; 3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium salt (XTT); dichloroindophenol (DCPIP); reactive oxygen species (ROS); regeneration; scavenging; chloroplast
类别
资金
- U.S. Department of Energy [ER46488]
- National Defense Science and Engineering Graduate (NDSEG) Fellowship
- TUBITAK
- METU-DPT-OYP program
- National Science Foundation (NSF) [1103600]
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1103600] Funding Source: National Science Foundation
The chloroplast contains densely stacked arrays of light-harvesting proteins that harness solar energy with theoretical maximum glucose conversion efficiencies approaching 12%. Few studies have explored isolated chloroplasts as a renewable, abundant, and low cost source for solar energy harvesting. One impediment is that photoactive proteins within the chloroplast become photodamaged due to reactive oxygen species (ROS) generation. In vivo, chloroplasts reduce photodegradation by applying a self-repair cycle that dynamically replaces photodamaged components; outside the cell, ROS-induced photodegradation contributes to limited chloroplast stability. The incorporation of chloroplasts into synthetic, light-harvesting devices will require regenerative ROS scavenging mechanisms to prolong photoactivity. Herein, we study ROS generation within isolated chloroplasts extracted from Spinacia oleracea directly interfaced with nanoparticle antioxidants, including dextran-wrapped nanoceria (dNC) previously demonstrated as a potent ROS scavenger. We quantitatively examine the effect of dNC, along with cerium ions, fullerenol, and DNA-wrapped single-walled carbon nanotubes (SWCNTs), on the ROS generation of isolated chloroplasts using the oxidative dyes, 2',7'- dichlorodihydrofluorescein diacetate (H2DCF-DA) and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide sodium salt (XTT). Electrochemical measurements confirm that chloroplasts processed from free solution can generate power under illumination. We find dNC to be the most effective of these agents for decreasing oxidizing species and superoxide concentrations whilst preserving chloroplast photoactivity at concentrations below 5 M, offering a promising mechanism for maintaining regenerative chloroplast photoactivity for light-harvesting applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据