期刊
ACS COMBINATORIAL SCIENCE
卷 16, 期 7, 页码 321-327出版社
AMER CHEMICAL SOC
DOI: 10.1021/co5000272
关键词
aptamers; SELEX (systematic evolution of ligands by exponential enrichment); C-reactive protein; magnetic nanoparticle; MARAS
资金
- National Science Council of Taiwan [102-2221-E-005-054]
A new SELEX protocol for the development of DNA aptamers has been demonstrated, referred to as magnetic-assisted rapid aptamer selection (MARAS). This method uses magnetic beads and an externally applied rotating magnetic field to provide the competitive mechanism for the selection aptamers with different affinities to the molecular target. The MARAS protocol efficiently generated aptamers with high affinity and specificity for C-reactive protein, a common cardiovascular disease indicator. The binding affinities of the selected aptamers could be varied by changing the frequency of the externally applied rotating magnetic field and optimal cases bound with low-nanomolar dissociation constants.
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