期刊
NATURE COMMUNICATIONS
卷 5, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms4052
关键词
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资金
- X-ray Free Electron Laser Priority Strategy Program from the Ministry of Education, Sports, Culture, Science and Technology, Japan (MEXT)
- CREST from the Japan Science and Technology Agency (JST)
- KAKENHI from Japan Society for the Promotion of Science (JSPS) [23651126, 22310075, 22540424, 23860001]
- Cooperative Research Program of 'Network Joint Research Center for Materials and Devices'
- Grants-in-Aid for Scientific Research [23860001, 22540424, 25400438, 22310075, 23651126] Funding Source: KAKEN
Emerging X-ray free-electron lasers with femtosecond pulse duration enable single-shot snapshot imaging almost free from sample damage by outrunning major radiation damage processes. In bioimaging, it is essential to keep the sample close to its natural state. Conventional high-resolution imaging, however, suffers from severe radiation damage that hinders live cell imaging. Here we present a method for capturing snapshots of live cells kept in a micro-liquid enclosure array by X-ray laser diffraction. We place living Microbacterium lacticum cells in an enclosure array and successively expose each enclosure to a single X-ray laser pulse from the SPring-8 Angstrom Compact Free-Electron Laser. The enclosure itself works as a guard slit and allows us to record a coherent diffraction pattern from a weakly-scattering submicrometre-sized cell with a clear fringe extending up to a 28-nm full-period resolution. The reconstructed image reveals living whole-cell structures without any staining, which helps advance understanding of intracellular phenomena.
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