期刊
NATURE COMMUNICATIONS
卷 5, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms6627
关键词
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资金
- National Basic Research Program of China [2012CB966902, 2010CB529605, 2011CB504400]
- National Natural Science Foundation of China [91132306, 81000551]
- Instrument Developing Project [2010019]
- Strategic Priority ResearchProgram (B) of the Chinese Academy of Sciences [XDB02050003]
- Guangdong Innovation Research Team Fund for Low-cost Healthcare Technologies
- Shenzhen Peacock Plan [KQCX20130628112914294]
- Shenzhen Governmental Basic Research [JC201006040897A, JC201005270296A, JC201005270291A]
Astrocytes provide neuroprotective effects against degeneration of dopaminergic (DA) neurons and play a fundamental role in DA differentiation of neural stem cells. Here we show that light illumination of astrocytes expressing engineered channelrhodopsin variant (ChETA) can remarkably enhance the release of basic fibroblast growth factor (bFGF) and significantly promote the DA differentiation of human embryonic stem cells (hESCs) in vitro. Light activation of transplanted astrocytes in the substantia nigra (SN) also upregulates bFGF levels in vivo and promotes the regenerative effects of co-transplanted stem cells. Importantly, upregulation of bFGF levels, by specific light activation of endogenous astrocytes in the SN, enhances the DA differentiation of transplanted stem cells and promotes brain repair in a mouse model of Parkinson's disease (PD). Our study indicates that astrocyte-derived bFGF is required for regulation of DA differentiation of the stem cells and may provide a strategy targeting astrocytes for treatment of PD.
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