期刊
NATURE COMMUNICATIONS
卷 5, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms4828
关键词
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资金
- Wellcome Trust Principal Research Fellowship
- MRC
- Wellcome Trust/MRC Strategic Grant in Neurodegeneration
- Wellcome Trust Strategic Grant
- NIHR Biomedical Research Unit at Addenbrooke's Hospital
- Wellcome Trust
- The Cambridge Overseas Trust
- MRC [MR/L007533/1, G0701444, G0700750, MC_G1000734] Funding Source: UKRI
- Medical Research Council [MC_G1000734, G0700750, MR/L007533/1, G0701444] Funding Source: researchfish
Endosomal protein sorting controls the localization of many physiologically important proteins and is linked to several neurodegenerative diseases. VPS35 is a component of the retromer complex, which mediates endosome-to-Golgi retrieval of membrane proteins such as the cation-independent mannose 6-phosphate receptor. Furthermore, retromer is also required for the endosomal recruitment of the actin nucleation promoting WASH complex. The VPS35 D620N mutation causes a rare form of autosomal-dominant Parkinson's disease (PD). Here we show that this mutant associates poorly with the WASH complex and impairs WASH recruitment to endosomes. Autophagy is impaired in cells expressing PD-mutant VPS35 or lacking WASH. The autophagy defects can be explained, at least in part, by abnormal trafficking of the autophagy protein ATG9A. Thus, the PD-causing D620N mutation in VPS35 restrictsWASH complex recruitment to endosomes, and reveals a novel role for the WASH complex in autophagosome formation.
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