期刊
NATURE COMMUNICATIONS
卷 5, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms6711
关键词
-
资金
- ISF [1193/09, 1096/13]
- Karen Siem Fellowship
- ERC [StG 260395]
It is well established that import of proteins into mitochondria can occur after their complete synthesis by cytosolic ribosomes. Recently, an additional model was revived, proposing that some proteins are imported co-translationally. This model entails association of ribosomes with the mitochondrial outer membrane, shown to be mediated through the ribosomeassociated chaperone nascent chain-associated complex (NAC). However, the mitochondrial receptor of this complex is unknown. Here, we identify the Saccharomyces cerevisiae outer membrane protein OM14 as a receptor for NAC. OM14 Delta mitochondria have significantly lower amounts of associated NAC and ribosomes, and ribosomes from NAC[Delta] cells have reduced levels of associated OM14 Delta Importantly, mitochondrial import assays reveal a significant decrease in import efficiency into OM14D mitochondria, and OM14-dependent import necessitates NAC. Our results identify OM14 as the first mitochondrial receptor for ribosome- associated NAC and reveal its importance for import. These results provide a strong support for an additional, co-translational mode of import into mitochondria.
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