期刊
NATURE COMMUNICATIONS
卷 2, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms1153
关键词
-
资金
- Gene Manipulation Facility of the Children's Hospital Mental Retardation and Developmental Disabilities Research Center (IDDRC) [NIHP30-HD18655]
- National Institutes of Health NICHD [2 U01 HD045857-06]
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [U01HD045857, P30HD018655] Funding Source: NIH RePORTER
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT [R01HD045339] Funding Source: NIH RePORTER
Calcium signalling is critical for successful fertilization. In spermatozoa, capacitation, hyperactivation of motility and the acrosome reaction are all mediated by increases in intracellular Ca2+. Cation channels of sperm proteins (CATSPERS1-4) form an alkalinization-activated Ca2+-selective channel required for the hyperactivated motility of spermatozoa and male fertility. Each of the CatSper1-4 genes encodes a subunit of a tetramer surrounding a Ca2+-selective pore, in analogy with other six-transmembrane ion channel a subunits. In addition to the pore-forming proteins, the sperm Ca2+ channel contains auxiliary subunits, CATSPER beta and CATSPER gamma. Here, we identify the Tmem146 gene product as a novel subunit, CATSPER delta, required for CATSPER channel function. We find that mice lacking the sperm tail-specific CATSPERd are infertile and their spermatozoa lack both Ca2+ current and hyperactivated motility. We show that CATSPERd is an essential element of the CATSPER channel complex and propose that CATSPERd is required for proper CATSPER channel assembly and/or transport.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据