期刊
NATURE COMMUNICATIONS
卷 2, 期 -, 页码 -出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ncomms1424
关键词
-
资金
- Wellcome Trust
- FEBS Short-Term Fellowship
- Foundation for Polish Science
- AstraZeneca
- EPSRC
- BBSRC
- Oxford MSc Programme in Clinical Embryology
- UK Medical Research Council [MC_US_A030_0022]
- EPSRC [EP/H004025/1] Funding Source: UKRI
- MRC [MC_U105261167, G0300284, G0800784] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/H004025/1] Funding Source: researchfish
- Medical Research Council [G0300284, G0800784B, MC_U105261167, G0800784] Funding Source: researchfish
Fertilization-induced cytoplasmic flows are a conserved feature of eggs in many species. However, until now the importance of cytoplasmic flows for the development of mammalian embryos has been unknown. Here, by combining a rapid imaging of the freshly fertilized mouse egg with advanced image analysis based on particle image velocimetry, we show that fertilization induces rhythmical cytoplasmic movements that coincide with pulsations of the protrusion forming above the sperm head. We find that these movements are caused by contractions of the actomyosin cytoskeleton triggered by Ca2+ oscillations induced by fertilization. Most importantly, the relationship between the movements and the events of egg activation makes it possible to use the movements alone to predict developmental potential of the zygote. In conclusion, this method offers, thus far, the earliest and fastest, non-invasive way to predict the viability of eggs fertilized in vitro and therefore can potentially improve greatly the prospects for IVF treatment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据