期刊
ONCOLOGY LETTERS
卷 7, 期 6, 页码 2192-2198出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2014.2048
关键词
breast cancer; tumor marker; circulating cancer cells; nested polymerase chain reaction
类别
资金
- Hebei Provincial Population and Family Planning Commission [2009-B21]
- Hebei Provincial Bureau of Health [20090579]
The aim of the present study was to develop a simple and rapid method for the detection of circulating cancer cells using multiple tumor markers and to investigate the clinical significance of circulating cancer cells. in breast cancer patients. A novel rapid nested polymerase chain reaction (PCR) assay, with high sensitivity and specificity, was evaluated, which was considered to be suitable for clinical application. The rapid nested PCR method was used to detect the circulating cancer cells of 142 breast cancer patients, using a panel of marker genes (FAM83A, NPY1R and KRT19), which were identified by the Digital Gene Expression Displayer Tool of the National Cancer Institute-Cancer Genome Anatomy Project. In total, 79.6% of the 142 breast cancer patient blood samples were found to express at least one tumor marker. In addition, the number of positive markers was found to significantly correlate with the disease stage and presence of distant metastasis. Furthermore, positivity for more than one tumor marker appeared to predict a reduced survival time in breast cancer patients.
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