4.4 Article

Elevated expression of solute carrier family 22 member 18 increases the sensitivity of U251 glioma cells to BCNU

期刊

ONCOLOGY LETTERS
卷 2, 期 6, 页码 1139-1142

出版社

SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2011.371

关键词

solute carrier family 22 member 18; apoptosis; glioma; BCNU

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资金

  1. Natural Science Foundation of China [30901535]
  2. Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine [10XHJT01]
  3. Shanghai Jiao Tong University School of Medicine [10XBR01]

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Previous studies showed that solute carrier family 22 member 18 (SLC22A18) is involved in tumorigenesis. The aim of this study was to examine the role of SLC22A18 in glioma cells. Glioma U251 cells were transfected with the human SLC22A18 gene. Transfection of the empty vector pcDNA3.1 was used as a negative control. Sensitivity to BCNU was measured by Annexin V staining. The expression of caspase-3 and bcl-2 was determined by immunohistochemistry. The transfection was confirmed by PCR, RT-PCR and Western blotting. Augmented apoptotic cell death was observed in the SLC22A18-transfected cells, compared to the non-transfected cells or cells with the empty vector. Caspase-3 expression increased in U251-SLC22A18 cells, whereas the bcl-2 expression decreased. These results indicated that SLC22A18 has a pro-apoptotic function in glioma cells.

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