期刊
EXPERIMENTAL AND THERAPEUTIC MEDICINE
卷 8, 期 2, 页码 614-622出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/etm.2014.1738
关键词
acute respiratory distress syndrome; CD11b/CD18; intercellular adhesion molecule-1; nuclear factor-kappa B; pyrrolidine dithiocarbamate
资金
- Project of Science and Technology Department of Liaoning Province [2013225305]
The aim of the present study was to investigate the regulatory mechanism of nuclear factor (NF)-kappa B on polymorphonuclear neutrophil (PMN) accumulation and the inflammatory response in lung tissues with acute respiratory distress syndrome (ARDS), as well as the therapeutic effect of pyrrolidine dithiocarbamate (PDTC). Mouse models of ARDS were established by intraperitoneal injection of lipopolysaccharide (LPS). BALB/c mice were divided into control, LPS and PDTC + LPS groups. The expression of PMN adhesion molecules, CD11b/CD18 and intercellular adhesion molecule-1 (ICAM-1), were detected by immunohistochemistry, while the protein expression levels of NF-kappa B p65 in the lung tissue were analyzed by western blot analysis. In addition, flow cytometry was used to investigate the apoptosis rate of PMNs in the bronchoalveolar fluid, and the expression levels of interleukin (IL)-1 beta, IL-8 and tumor necrosis factor (TNF)-alpha and myeloperoxidase (MPO) activity were also determined. Following an intraperitoneal injection of LPS, alveolar septum rupture, pulmonary interstitial hyperemia and PMN infiltration in the alveolar was observed. The protein expression of p65 in the pulmonary cytoplasm decreased, while the expression of p65 in the nucleus increased. The levels of IL-8, IL-1 beta and TNF-alpha increased and the high expression status was maintained for 24 h. As the time increased, CD11b/CD18 and ICAM-1 expression increased, as well as MPO activity, while the apoptosis of PMNs was delayed. Compared with the LPS group, the expression of p65 in the pulmonary cytoplasm and the PMN apoptosis rate increased following PDTC intervention, while the expression of p65 in the nucleus decreased, as well as the expression levels of the cytokines and MPO activity. Therefore, PDTC reduced the production of inflammatory cytokines via the NF-kappa B pathway, which reduced the activation of PMNs in the lung tissue and promoted PMN apoptosis.
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