期刊
INTERNATIONAL JOURNAL OF ORAL SCIENCE
卷 3, 期 1, 页码 34-42出版社
NATURE PUBLISHING GROUP
DOI: 10.4248/IJOS11014
关键词
honokiol; oral squamous cell carcinoma; anticancer; apoptosis
资金
- National Science Funds for Talented Professionals of China [30725041]
- National Natural Science Foundation of China [30930100, 30672323, 81072218]
- State Key Laboratory of Oral Diseases Open Funding of China [SKLODOF 2010-01]
- Ministry of Education, China
Honokiol (HNK) is a small organic molecule purified from magnolia species and has demonstrated anticancer activities in a variety of cancer cell lines; however, its effect on oral squamous cell carcinoma (OSCC) cells is unknown. We investigated the antitumor activities of HNK on OSCC cells in vitro for the first time. The inhibitory effects of HNK on the growth and proliferation of OSCC cells were demonstrated via in vitro 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and propidium iodide (PI) assays, and the apoptotic cells were investigated by the observation of morphological changes and detection of DNA fragmentation via PI, TdT-mediated dUTP-biotin nick end labeling (TUNEL), and DNA ladder assays, as well as flow cytometry assay. The results showed that HNK inhibited the growth and proliferation of OSCC cells in vitro in a time and dose-dependent manner. The inhibitory effect was associated with the cell apoptosis induced by LINK, evidenced by the morphological features of apoptotic cells, TUNEL-positive cells and a degradation of chromosomal DNA into small internucleosomal fragments. The study also demonstrated here that the inhibition or apoptosis mediated by 15 mu g.mL(-1) or 20 mu g.mL(-1) of HNK were more stronger compared with those of 20 mu g.mL(-1) 5-fluorouracil (5-Fu, the control) applied to OSCC cells, when the ratio of OSCC cell numbers were measured between the treatment of different concentrations of HNK to the 5-Fu treatment for 48 h. HNK is a promising compound that can be potentially used as a novel treatment agent for human OSCC.
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