期刊
AUTOPHAGY
卷 11, 期 12, 页码 2259-2274出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15548627.2015.1113365
关键词
ATG6; chloroplast autophagy; leaf starch degradation; macroautophagy; microtubules; plants
类别
资金
- National Basic Research Program of China [2011CB910100]
- National Natural Science Foundation of China [3142100007, 31470254, 31270182]
- China Postdoctoral Science Foundation [2014M550048]
- Specialized Research Fund for the Doctoral Program of Higher Education of China [20120002130005]
- National Transgenic Program of China [2014ZX08009-003, 2014ZX08005001]
- Postdoctoral Fellowship of Tsinghua-Peking Joint Center for Life Sciences
Microtubules, the major components of cytoskeleton, are involved in various fundamental biological processes in plants. Recent studies in mammalian cells have revealed the importance of microtubule cytoskeleton in autophagy. However, little is known about the roles of microtubules in plant autophagy. Here, we found that ATG6 interacts with TUB8/-tubulin 8 and colocalizes with microtubules in Nicotiana benthamiana. Disruption of microtubules by either silencing of tubulin genes or treatment with microtubule-depolymerizing agents in N. benthamiana reduces autophagosome formation during upregulation of nocturnal or oxidation-induced macroautophagy. Furthermore, a blockage of leaf starch degradation occurred in microtubule-disrupted cells and triggered a distinct ATG6-, ATG5- and ATG7-independent autophagic pathway termed starch excess-associated chloroplast autophagy (SEX chlorophagy) for clearance of dysfunctional chloroplasts. Our findings reveal that an intact microtubule network is important for efficient macroautophagy and leaf starch degradation.
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