期刊
JOURNAL OF MOLECULAR CELL BIOLOGY
卷 5, 期 4, 页码 227-238出版社
OXFORD UNIV PRESS
DOI: 10.1093/jmcb/mjt011
关键词
extracellular vesicles; genomic DNA; AT(1) receptor; BCR; ABL hybrid gene
类别
资金
- National Natural Science Foundation of China [30925018, 31130029]
- National Basic Research Program of China (973 Program) [2008\CB517308, 2012CB517801, 2013CB531104]
- Natural Science Foundation Project of CQ CSTC (CSTC) [2009BA5044]
- National Institutes of Health, USA [R37HL023081, P01HL074940]
Extracellular vesicles (EVs) carry signals within or at their limiting membranes, providing a mechanism by which cells can exchange more complex information than what was previously thought. In addition to mRNAs and microRNAs, there are DNA fragments in EVs. Solexa sequencing indicated the presence of at least 16434 genomic DNA (gDNA) fragments in the EVs from human plasma. Immunofluorescence study showed direct evidence that acridine orange-stained EV DNAs could be transferred into the cells and localize to and inside the nuclear membrane. However, whether the transferred EV DNAs are functional or not is not clear. We found that EV gDNAs could be homologously or heterologously transferred from donor cells to recipient cells, and increase gDNA-coding mRNA, protein expression, and function (e.g. AT(1) receptor). An endogenous promoter of the AT(1) receptor, NF-B, could be recruited to the transferred DNAs in the nucleus, and increase the transcription of AT(1) receptor in the recipient cells. Moreover, the transferred EV gDNAs have pathophysiological significance. BCR/ABL hybrid gene, involved in the pathogenesis of chronic myeloid leukemia, could be transferred from K562 EVs to HEK293 cells or neutrophils. Our present study shows that the gDNAs transferred from EVs to cells have physiological significance, not only to increase the gDNA-coding mRNA and protein levels, but also to influence function in recipient cells.
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