期刊
JOURNAL OF MOLECULAR CELL BIOLOGY
卷 1, 期 1, 页码 46-54出版社
OXFORD UNIV PRESS
DOI: 10.1093/jmcb/mjp003
关键词
pluripotency; reprogram; iPS cells; pig; ungulate; embryonic stem cells
类别
资金
- National Key Basic Research and Development Program of China [2007 CB948003, 2009CB940900, 2009CB941000, 2009CB941100]
- National Natural Science Foundation of China [30621091]
- Chinese Academy of Sciences [KSCX1-YW-R-54, KSCX1-YW-R-46]
- Shanghai Science & Technology Developmental Foundation [08DJ1400500]
- Shanghai Institutes for Biological Sciences [2007KIP401]
Domesticated ungulate pluripotent embryonic stem (ES) cell lines would be useful for generating precise gene-modified animals. To date, many efforts have been made to establish domesticated ungulate pluripotent ES cells from early embryos without success. Here, we report the generation of porcine-induced pluripotent stem (iPS) cells using drug-inducible expression of defined factors. We showed that porcine iPS cells expressed alkaline phosphatase, SSEA3, SSEA4, Tra-1-60, Tra-1-81, Oct3/4, Nanog, Sox2, Rex1 and CDH1. Pig iPS cells expressed high levels of telomerase activity and showed normal karyotypes. These cells could differentiate into cell types of all three germ layers in vitro and in teratomas. Our study reveals properties of porcine pluripotent stem cells that may facilitate the eventual establishment of porcine ES cells. Moreover, the porcine iPS cells produced may be directly useful for the generation of precise gene-modified pigs.
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