期刊
SURGICAL INFECTIONS
卷 20, 期 1, 页码 16-24出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/sur.2018.135
关键词
bacteriophage; biofilm; implant; prosthetic joint infection; Staphylococcus aureus
资金
- Orthopaedic Research Institute of Queensland
- James Cook University
Background: Despite significant advancements in surgical protocols and biomaterials for orthopedics, periprosthetic joint infection (PJI) remains a leading cause of implant failure. Staphylococcus aureus nasal colonization is an established risk factor for PJI, with methicillin-sensitive S. aureus a leading cause of orthopedic implant-related infections. The purpose of these in vitro studies was to investigate the antibacterial activity of a tailored bacteriophage cocktail against planktonic and biofilm-associated S. aureus. Methods: The S. aureus strains (n = 30) were screened for their susceptibility to a library of S. aureus-specific bacteriophage (n = 31). Five bacteriophage preparations that demonstrated bactericidal activity against > 90% of S. aureus strains tested were combined as a StaPhage cocktail and assessed for their antibacterial activity toward planktonic and biofilm-associated S. aureus, with biofilms established on three-dimensional-printed porous titanium scaffolds. Results: StaPhage treatment immediately after bacterial inoculation inhibited growth of S. aureus by > 98% in eight hour cultures when multiplicity of infection of phages to bacteria was greater than 1: 1 (p < 0.01). Viable bacterial numbers within biofilms on titanium surfaces were significantly reduced (6.8 log(10) to 6.2 log(10) colony forming units [CFU]; p < 0.01) after exposure to the StaPhage cocktail, in vitro. No significant reduction was observed in biofilms exposed to 100 times the minimal inhibitory concentration of cefazolin (log10 6.81 CFU). Conclusions: Combined, these data demonstrate the in vitro efficacy of S. aureus-specific bacteriophage cocktails against S. aureus growing on porous titanium and warrant further in vivo studies in a clinically relevant animal model to evaluate the potential application of bacteriophage in the management of PJI caused by S. aureus.
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