期刊
MINE WATER AND THE ENVIRONMENT
卷 32, 期 4, 页码 258-265出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10230-013-0250-8
关键词
Microbial iron oxidation; Live/dead-stain; Microbial community analysis; Ferrovum; Saxony; Germany
资金
- Federal Ministry of Education and Research (BMBF) within the Geotechnologien RD program [03G0714B]
Treatment of acidic iron-and sulfate-rich mine waters in a pilot plant at the opencast lignite mining pit Nochten (Lusatia, Germany) involves microbial iron oxidation and subsequent precipitation of the iron-oxyhydroxysulfate schwertmannite. To determine if recirculation of schwertmannite can stabilize and optimize the oxidation process, cell number, viability, and diversity of the bacterial community in schwertmannite precipitated on carrier material and stored schwertmannitewere analyzed. In schwertmannite on carrier material, the total cell number decreased slightly with increasing mineral depth, whereas the percentage of viable cells decreased significantly. The microbial community, investigated by fluorescence-in-situ-hybridization (FISH) and terminal restriction fragment length polymorphism (T-RFLP), revealed the presence of the iron-oxidizing bacterium Ferrovum sp. and relatives of Gallionella, independent of mineral depth. Analysis of the viability of microorganism in stored schwertmannite indicated an almost constant percentage of viable cells over 42 days.
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