期刊
JOURNAL OF FUNCTIONAL FOODS
卷 48, 期 -, 页码 594-604出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2018.07.060
关键词
Antioxidant peptide; Oxidative stress; Reactive oxygen species; Purple sea urchin Strongylocentrotus nudus; Caenorhabditis elegans
资金
- Guangdong Province Department of Education [2015KGJHZ022]
- Guangdong Provincial Medical Science Foundation [A2016232]
- climbing program of Guangdong Province Students for Scientific and Technological Innovation [138981]
- Special Funds of the Central Finance
The papain hydrolyzate prepared from purple sea urchin (Strongylocentrotus nudus) gonad exerts significant antioxidant activity. Through ultrafiltration, gel filtration and high performance liquid chromatography, the active fractions from the hydrolyzate were screened by alleviating paraquat-induced oxidative stress in Caenorhabditis elegans, and the peptide components were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Two antioxidant peptides SnP7 (AAVPSGASTGIYEALELR, 1805.03 Da) and SnP10 (NPLLEAFGNAK, 1173.34 Da) exhibit significant antioxidant capacity, which reduce reactive oxygen species (ROS) level and the expression of superoxide dismutase-3 (SOD-3) and heat shock protein-16.2 (HSP-16.2) in oxidation-damaged nematodes. Further studies indicated that SnP7 and SnP10 induce DAF-16 nuclear translocation and the expression of stress-related genes such as sod-3. RNA interference (RNAi) suggested that peptide induced enhancement of resistance is dependent on DAF-16. Taken together, S. nudus antioxidant peptides might act through activate DAF-16 signaling pathway, and induce the expression of DAF-16 target genes that enhance antioxidant potential of organisms.
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