期刊
JOURNAL OF FUNCTIONAL FOODS
卷 11, 期 -, 页码 142-151出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2014.09.011
关键词
Black currant extract; Antioxidant activity; Protective effects; Hydrogen peroxide-induced; damage; MRC-5 cells
资金
- National Natural Science Foundation of China [31301509]
- National 12th Five-Year Science and Technology supporting plan in China [2012BAD28B02]
A black currant (Ribes nigrum L.) extract (BCE) was prepared to investigate its protective effects against damage to lung fibroblast MRC-5 cells induced by H2O2. BCE exhibited strong scavenging activities against hydroxyl (center dot OH) and superoxide anion radicals (O-2(center dot-)) in vitro. Exposing MRC-5 cells to 11202 reduced the cell viability; the activities of antioxidant enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), and cell apoptosis while increasing the thiobarbituric acid reactive substances (TBARS) content. Pretreating cells with different concentrations of BCE (0, 2.5, 5 and 10 mg/ml) enhanced the cell viability and antioxidant enzyme activities while decreasing the TBARS content. Morphological observations and flow cytometric analysis revealed that BCE alleviated the cell apoptosis induced by H2O2. These protective effects of BCE on MRC-5 cells suggest that black currants may possess the ability to eliminate over-produced ROS in humans. (C) 2014 Elsevier Ltd. All rights reserved.
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