期刊
JOURNAL OF FUNCTIONAL FOODS
卷 7, 期 -, 页码 287-297出版社
ELSEVIER
DOI: 10.1016/j.jff.2014.01.032
关键词
Citrus aurantium L; Non-Small Cell Lung Cancer (NSCLC); Flavonoids; PKH-26; Metastasis
资金
- National Research Foundation (NRF) of Korea Grant - Korean government (MEST) [2012045015]
- National R&D Program for Cancer Control, Ministry for Health, Welfare and Family affairs, Republic of Korea [0820050]
- National Research Foundation of Korea [2012M3A9B8019303, 2009-0062556, 2012R1A2A2A06045015] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Korean Citrus aurantium L. has long been used as a traditional medicine for the treatment of various cancers such as those of lung and prostat. C. aurantium L. has been reported to have anti-metastasis properties in in vitro experiments. However, the anti-metastasis effect of C. aurantium L. in vivo is not well understood. This study investigated the anti-metastatic effect of flavonoids isolated from Korean C. aurantium L. using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) in NOD/SCID mice. PKH-26 staining and immunohistochemical analyses indicated that flavonoids prevented cancer cell infiltration and localization to the lung. Also, flavonoids induced cancer cell apoptosis through the regulation of the apoptosis related protein cleaved caspase-3 and p-p53. In vitro results also show that flavonoids inhibited A549 cells metastasis and induced apoptosis. Moreover, proteomic analysis indicated comparable down-regulation of the Ddx3x and ANP43B proteins with control group after treatment with flavonoids. These results provide scientific support for the use of flavonoids isolated from C. aurantium L. for the treatment of human lung cancer. (C) 2014 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据