期刊
JOURNAL OF FUNCTIONAL FOODS
卷 5, 期 1, 页码 337-345出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jff.2012.11.004
关键词
Myricitrin; Lipid peroxidation; Mitochondria; Apoptosis
资金
- Young Foundation in CAMS PUMC
- National Science and Technology Major Project [2012ZX09103101-078]
- research special fund for public welfare industry of health [200902008]
Parkinson's disease (PD) is a neurodegenerative disorder characterized by progressive loss of dopaminergic (DA) neurons at the substantia nigra. 6-Hydroxydopamine (6-OHDA) is a dopamine analog, which specifically to damage dopaminergic neurons. Myricitrin, a flavanoid isolated from the root bark of Myrica cerifera, has antinociceptive activity, anti-inflammatory, antioxidant, and immunomodulatory properties. In the present study, the potential protection and mechanism of myricitrin against 6-OHDA-induced damage and apoptosis in PC12 cells was studied. The results showed that myricitrin attenuated 6-OHDA-induced cell damage and mitochondrial dysfunction in a dose-dependent manner, which was correlated with decreased intracellular ATP content and mitochondrial membrane potential. Furthermore, it was found that myricitrin inhibited the apoptosis of PC12 cells induced by 6-OHDA in relation to reduction of cytochrome C release from mitochondria and inhibition of the activity of caspase-3. Finally, the antioxidation of myricitrin in PC12 cells and brain mitochondria was investigated. The results showed that myricitrin decreased the production of reactive oxygen species in PC12 cells and inhibited lipid peroxidation in rat brain mitochondria (IC50 = 3.19 +/- 0.34 mu M). Thus, myricitrin has the neuroprotective capacity to antagonize 6-OHDA-induced neurotoxicity in PC12 cells and may be useful in treating PD. (C) 2012 Elsevier Ltd. All rights reserved.
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