期刊
CONSERVATION LETTERS
卷 6, 期 6, 页码 402-409出版社
WILEY
DOI: 10.1111/conl.12017
关键词
Carcinus maenas; Dreissena; zebra mussel; quagga mussel; Eriocheir sinensis; Limnoperna fortunei
资金
- Great Lakes Protection Fund
- U. S. Environmental Protection Agency
- University of Notre Dame's Environmental Change Initiative and its Advanced Diagnostics & Therapeutics Initiative
Invasive aquatic species introductions cause tremendous environmental and economic damage. Conservation and management efforts will benefit from rapid, inexpensive, and accurate on-site methods to detect harmful aquatic species to prevent their introduction and spread. Here, two technologies, environmental DNA (eDNA) sampling and Light Transmission Spectroscopy (LTS), were combined to address this need. Specifically, eDNA filtering and extraction methods were used to isolate DNA from: (1) lake water samples that were seeded with a microscopic fragment of five high-risk invasive species and (2) untreated samples from lakes infested with the invasive zebra mussel, Dreissena polymorpha, followed by polymerase chain reaction (PCR) amplification. LTS was then used to detect size shifts resulting from hybridization of PCR products with nanobeads covered with species-specific oligonucleotide probes. The results demonstrate that coupling eDNA sampling with LTS species detection can provide a sensitive and real-time solution for screening real-world water samples for invasive species.
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