期刊
MEDCHEMCOMM
卷 5, 期 3, 页码 277-287出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3md00276d
关键词
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资金
- JSPS KAKENHI
- Program for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry
- Grants-in-Aid for Scientific Research [25350981] Funding Source: KAKEN
Identification of the cellular targets of bioactive small molecules is a crucial step in drug discovery and chemical genetics. Classical affinity purification with small-molecule affinity probes remains the most common approach, but it remains a considerable challenge. To overcome various drawbacks in probe preparation, nonspecific interactions, and the sensitivity of target detection, new methods and techniques are being developed, such as a nonselective universal coupling method based on a photo-affinity reaction, which enables introduction of a variety of small molecules to a solid support without chemical modification to generate small-molecule affinity probes. Here, we review recent progress in affinity-based approaches for small-molecule target identification with a focus on affinity purification.
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