期刊
FRONTIERS IN NEURAL CIRCUITS
卷 7, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fncir.2013.00151
关键词
acousto-optical deflectors; two-photon imaging; motion-tracking; correlation structure; microcircuit activity; population coding
资金
- National Eye Institute-United States National Institutes of Health (NIH, NEI, NIDA, NIMH) [5R01DA028525, 5-T32-EY07001-37, 5-P30-EY002520-33]
- National Eye Institute-United States National Institutes of Health (NIH-Pioneer award) [DPI EY023176]
- McKnight Scholar Award
- Arnold and Beckman Foundation Young Investigator Award
- NIMH [F30 MH088228-04]
Great progress has been made toward under standing the properties of single neurons, yet the principles underlying interactions between neurons remain poorly understood. Given that connectivity in the neocortex is locally dense through both horizontal and vertical connections, it is of particular importance to characterize the activity structure of local populations of neurons arranged in three dimensions. However, techniques for simultaneously measuring microcircuit activity are lacking. We developed an in vivo 3D high-speed, random-access two-photon microscope that is capable of simultaneous 3D motion tracking. This allows imaging from hundreds of neurons at several hundred Hz, while monitoring tissue movement. Given that motion will induce common artifacts across the population, accurate motion tracking is absolutely necessary for studying population activity with random-access based imaging methods. We demonstrate the potential of this imaging technique by measuring the correlation structure of large populations of nearby neurons in the mouse visual cortex, and find that the micro circuit correlation structure is stimulus dependent. Three-dimensional random access multiphoton imaging with con current motion tracking provides a novel, powerful method to characterize the microcircuit activity in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据