期刊
AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL
卷 193, 期 -, 页码 51-56出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.autneu.2015.07.002
关键词
Serotonin; Isoflurane; Anesthesia; Autonomic; Raphe
资金
- National Science Foundation [IOS 1022442]
- National Institutes of Health through awards from the National Institutes of Neurological Disorders and Stroke [2U54NS041069]
- National Institute of General Medical Sciences [SC2GM112570]
- Institutional Development Award (IDeA) from the National Institute of General Medical Sciences [P20GM103395]
Background: Anesthetics have a profound influence on a myriad of autonomic processes. Mechanisms of general anesthesia, and how these mechanisms give rise to the multifaceted state of anesthesia, are largely unknown. The ascending and descending serotonin (5-HT) networks are key modulators of autonomic pathways, and are critically involved in homeostatic reflexes across the motor, somatosensory, limbic and autonomic systems. These 5-HT networks are thought to contribute to anesthetic effects, but how anesthetics affect 5-HT neuron function remains a pertinent question. We hypothesized that the volatile anesthetic isoflurane inhibits action potential discharge of medullary raphe 5-HT neurons. Methods: We conducted extracellular recordings on individual neurons in the medullary raphe region of the unanesthetized in situ perfused brainstem preparation to determine how exposure to isoflurane affects 5-HT neurons. We examined changes in 5-HT neuron baseline firing in response to treatment with either 1, 1.5, or 2% isoflurane. We measured isoflurane concentrations by gas chromatography-mass spectrometry (GC-MS) analysis. Results: Exposure to isoflurane inhibited action potential discharge in raphe 5-HT neurons. We document a concentration-dependent inhibition over a range of concentrations approximating isoflurane MAC (minimum alveolar concentration required for surgical anesthesia). Delivered concentrations of isoflurane were confirmed using GC-MS analysis. Conclusions: These findings illustrate that halogenated anesthetics greatly affect 5-HT neuron firing and suggest 5-HT neuron contributions to mechanisms of general anesthesia. (C) 2015 Elsevier B.V. All rights reserved.
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