期刊
FRONTIERS IN CELLULAR NEUROSCIENCE
卷 8, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fncel.2014.00151
关键词
gaba receptors; homeostatic plasticity; axon initial segment; calcium; quantum dots; diffusion
资金
- BBSRC [BB/I00274X/1] Funding Source: UKRI
- MRC [G0802377] Funding Source: UKRI
- Medical Research Council [G0802377] Funding Source: Medline
- Medical Research Council [G0802377] Funding Source: researchfish
The axon initial segment (AIS), a site of action potential initiation, undergoes activity-dependent homeostatic repositioning to fine-tune neuronal activity. However, little is known about the behavior of GABA(A) receptors (GABA(A)Rs) at synapses made onto the axon and especially the AIS. Here, we study the clustering and lateral diffusion of GABA(A)Rs in the AIS under baseline conditions, and find that GABA(A)R lateral mobility is lower in the AIS than dendrites. We find differences in axonal clustering and lateral mobility between GABA(A)Rs containing the alpha 1 or alpha 2 subunits, which are known to localize differentially to the AIS. Interestingly, we find that chronic activity driving AIS repositioning does not alter GABAergic synapse location along the axon, but decreases GABA(A)R cluster size at the AIS. Moreover, in response to chronic depolarization, GABA(A)R diffusion is strikingly increased in the AIS, and not in dendrites, and this is coupled with a decrease in synaptic residency time of GABA(A)Rs at the AIS. We also demonstrate that activation of L-type voltage-gated calcium channels is important for regulating GABA(A)R lateral mobility at the AIS during chronic depolarization. Modulation of GABA(A)R diffusion dynamics at the AIS in response to prolonged activity may be a novel mechanism for regulating GABAergic control of information processing.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据