4.1 Article

Centrally administered isoproterenol induces sympathetic outflow via brain prostaglandin E2-mediated mechanisms in rats

期刊

出版社

ELSEVIER SCIENCE BV
DOI: 10.1016/j.autneu.2014.12.002

关键词

beta-Adrenoceptor; Prostaglandin E-2; Plasma noradrenaline; Hypothalamic paraventricular nucleus

资金

  1. Ministry of Education, Culture, Sports, Science, and Technology, Japan [24590903, S1101027]
  2. Grants-in-Aid for Scientific Research [24590903] Funding Source: KAKEN

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Brain beta-adrenoceptor stimulation can induce elevations of plasma levels of noradrenaline. However, there have been no detailed studies related to signaling pathways downstream of beta-adrenoceptors responsible for central sympathetic outflow. In the present study, we pharmacologically examined the possibility that centrally administered isoproterenol can induce elevations of plasma noradrenaline levels in a brain prostaglandin-dependent manner. In addition, we also examined whether or not intracerebroventricular administration of isoproterenol could release endogenously synthesized prostaglandin (PG) E-2 in the hypothalamic paraventricular nucleus (PVN) by using the brain microdialysis technique combined with liquid chromatography-ion trap tandem mass spectrometry (LC-ITMSn). Under urethane anesthesia, a femoral venous line was inserted for infusion of saline and a femoral arterial line was inserted for collecting blood samples. Next, animals were placed in a stereotaxic apparatus for application of test agents. Catecholamines in the plasma were extracted by alumina absorption and were assayed by high-performance liquid chromatography with electrochemical detection. Quantification of PGE(2) in rat PVN microdialysates was performed by the LC-ITMSn method. We demonstrated that centrally administered isoproterenol-induced elevations of plasma noradrenaline could be mediated via activation of beta-adrenoceptors and the downstream phospholipase A(2)-cyclooxygenase pathway. Furthermore, PGE(2) in the PVN and the PGE(2) receptor EP3 subtype appear to play an important role in the process. Our results suggest that central isoproterenol-induced sympathetic outflow is mediated via brain PGE(2) in a PGE(2) receptor EP3 subtype-dependent manner. (C) 2014 Elsevier B.V. All rights reserved.

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