期刊
CHEMICAL SCIENCE
卷 5, 期 4, 页码 1591-1598出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3sc51691a
关键词
-
资金
- National Science Foundation [CHE-0842590]
- National Cancer Institute of the U. S. National Institutes of Health [RO1 CA88986]
- National Institute of General Medical Sciences of the U. S. National Institutes of Health [R01 GM61761]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1213789] Funding Source: National Science Foundation
The selective derivatization of solvent-exposed cysteine residues in peptides and proteins is achieved by brief irradiation of an aqueous solution containing 3-(hydroxymethyl)-2-naphthol derivatives (NQMPs) with a 350 nm fluorescent lamp. NQMP can be conjugated with various moieties, such as PEG, dyes, carbohydrates, or possess a fragment for further selective derivatization, e. g., biotin, azide, alkyne, etc. Attractive features of this labeling approach include an exceptionally fast rate of the reaction and a requirement for a low equivalence of the reagent. The NQMP-thioether linkage is stable under ambient conditions, and survives protein digestion and MS analysis. Irradiation of an NQMP-labeled protein in a dilute solution (<40 mu M) or in the presence of a vinyl ether results in a traceless release of the substrate. The reversible biotinylation of bovine serum albumin, as well as the capture and release of this protein using NeutrAvidin Agarose resin beads has been demonstrated.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据