期刊
CHEMICAL SCIENCE
卷 2, 期 10, 页码 1952-1959出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1sc00120e
关键词
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资金
- Research Councils UK [GR/S79268/02]
- UK Engineering and Physical Sciences Research Council [EP/G037604/1]
- US National Institute of General Medical Sciences [GM62116]
- Royal Society
- BBSRC [BBS/E/J/000C0618, BB/E004350/1] Funding Source: UKRI
- EPSRC [EP/G026688/1, EP/E000614/1, EP/I500200/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/E004350/1, EGA17763, BB/C510824/1, BBS/E/J/000C0618] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/D023343/1, EP/G026688/1, EP/E000614/1, EP/I500200/1, GR/T26542/01, EP/D023335/1] Funding Source: researchfish
Carbohydrate microarrays provide access to high through-put analysis of protein-carbohydrate interactions. Herein we demonstrate the use of SPR imaging (SPRi) of glycoarrays to assess the ligand specificity of the reputedly galactose-specific plant lectin RCA(120) (Ricinus communis agglutinin 120), a surrogate for the bioterrorism agent ricin. Glycoarray studies identified RCA(120) ligands based on galactose substituted at the 6-position with sialic acid. These observations, which were confirmed by saturation transfer difference (STD) NMR spectroscopy studies, inspired the synthesis of non-natural 6-substituted galactose derivatives, which were shown to have similar to 3-4 fold enhanced binding to RCA(120) with respect to the unsubstituted compound. These novel unnatural galactosides, which are chemically and biologically more robust than their natural glycan counterparts, represent new potential ligands for the development of carbohydrate-based ricin sensors.
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