期刊
JOURNAL OF MATERIALS CHEMISTRY B
卷 3, 期 6, 页码 977-982出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4tb01625d
关键词
-
资金
- National Natural Science Foundation of China [21204055, 21334004, 21474071]
- National Natural Science Foundation Fund for Distinguished Young Scholars [21125418]
- Project of Scientific and Technologic Infrastructure of Suzhou [SZS201207]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
A major issue in the therapeutic use of tissue plasminogen activator (t-PA) for the treatment of thrombotic diseases is its very short half-life in the circulation due to the effects of inhibitors. The present study aims to resolve the issue using a t-PA/gold nanoparticle (t-PA/AuNP) conjugate prepared via bio-affinity ligation under physiological conditions. The ligation is based on the specific interactions between t-PA and epsilon-lysine (a ligand that has affinity to a specific domain in t-PA) immobilized on the AuNP surface through polyvinyl pyrrolidone (PVP) as a spacer. The conjugate can not only retain almost full enzymatic activity and clot dissolving efficiency, but also protect t-PA from inhibition by PAI-1 to some extent as compared with free t-PA in vitro. Moreover, the conjugate showed prolonged circulation time in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据