期刊
TICKS AND TICK-BORNE DISEASES
卷 4, 期 1-2, 页码 72-77出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.ttbdis.2012.07.006
关键词
Theileria annulata; Theileriosis; Real-time PCR; Tams1; Molecular diagnostics
资金
- Portuguese Fundacao para a Ciencia e a Tecnologia [SFRH/BD/64136/2009]
- European Deputy Rui Tavares
- Fundação para a Ciência e a Tecnologia [SFRH/BD/64136/2009] Funding Source: FCT
Tropical or Mediterranean theileriosis is a tick-borne hemoprotozoan disease that poses important health problems in cattle. The etiological agent is the apicomplexan parasite Theileria annulata that occurs around the Mediterranean basin, Middle East, and southern Asia. PCR-based assays have been developed for detecting theilerial infections, and the gene encoding an immunodominant major merozoite/piroplasm surface antigen of T. annulate (Tams1) is a commonly used genomic target. However, several studies revealed that this gene is highly polymorphic raising questions concerning the suitability of Tams1 genetargeted primers to detect all T. annulate isolates. In this work, we re-evaluated the in silico sensitivity of previously developed primers targeting the Tams1 gene, designed novel and more comprehensive primers for its amplification, and established efficient standard and real-time PCR assays for assessing the presence or absence of T. annulate in bovine blood samples. (C) 2012 Elsevier GmbH. All rights reserved.
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