期刊
VIRUSES-BASEL
卷 10, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/v10080446
关键词
human respiratory syncytial virus (RSV); cryo-electron tomography (cryo-ET); cryo-electron microscopy (cryo-EM); enveloped virus; virus assembly; viral morphogenesis
类别
资金
- Emory University
- Children's Healthcare of Atlanta
- Georgia Research Alliance
- Center for AIDS Research at Emory University [P30 AI050409]
- James B. Pendleton Charitable Trust
- NIH [R21 AI101775, R01 GM114561]
- NSF [0923395]
- United States Department of Veterans Affairs [2I01BX000624, K24 AI77930, T32 AI074492, K12 HD072245, F32 GM112517]
- Emory BCDB Training Grant [T32 GM008367-28]
- Emory Children's Center for Childhood Infections and Vaccines (CCIV)
- National Institute of Allergy and Infectious Diseases
- [R01 AI087798]
- [U19 AI095227]
- [R01 AI111820]
Human respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract disease in young children. With repeat infections throughout life, it can also cause substantial disease in the elderly and in adults with compromised cardiac, pulmonary and immune systems. RSV is a pleomorphic enveloped RNA virus in the Pneumoviridae family. Recently, the three-dimensional (3D) structure of purified RSV particles has been elucidated, revealing three distinct morphological categories: spherical, asymmetric, and filamentous. However, the native 3D structure of RSV particles associated with or released from infected cells has yet to be investigated. In this study, we have established an optimized system for studying RSV structure by imaging RSV-infected cells on transmission electron microscopy (TEM) grids by cryo-electron tomography (cryo-ET). Our results demonstrate that RSV is filamentous across several virus strains and cell lines by cryo-ET, cryo-immuno EM, and thin section TEM techniques. The viral filament length varies from 0.5 to 12 m and the average filament diameter is approximately 130 nm. Taking advantage of the whole cell tomography technique, we have resolved various stages of RSV assembly. Collectively, our results can facilitate the understanding of viral morphogenesis in RSV and other pleomorphic enveloped viruses.
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