期刊
VIRUSES-BASEL
卷 6, 期 9, 页码 3563-3583出版社
MDPI AG
DOI: 10.3390/v6093563
关键词
Adenovirus; virion stability; viral vectors; helper-dependent Ad
类别
资金
- Canadian Institutes of Health Research (CIHR)
- Cancer Research Society (Canada)
- National Sciences and Engineering Research Council (NSERC)
- Queen Elizabeth II Graduate Scholarship in Science and Technology from the Ontario Government
Adenovirus (Ad) vectors are currently the most commonly used platform for therapeutic gene delivery in human gene therapy clinical trials. Although these vectors are effective, many researchers seek to further improve the safety and efficacy of Ad-based vectors through detailed characterization of basic Ad biology relevant to its function as a vector system. Most Ad vectors are deleted of key, or all, viral protein coding sequences, which functions to not only prevent virus replication but also increase the cloning capacity of the vector for foreign DNA. However, radical modifications to the genome size significantly decreases virion stability, suggesting that the virus genome plays a role in maintaining the physical stability of the Ad virion. Indeed, a similar relationship between genome size and virion stability has been noted for many viruses. This review discusses the impact of the genome size on Ad virion stability and emphasizes the need to consider this aspect of virus biology in Ad-based vector design.
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