期刊
VIRUSES-BASEL
卷 6, 期 5, 页码 2028-2037出版社
MDPI
DOI: 10.3390/v6052028
关键词
Andes virus; Chile; ELISA; strip immunoblot assay; hantavirus
类别
资金
- FONDECYT [1110664]
- National Institute of Allergy and Infectious Diseases at the National Institutes of Health [5U19AI045452]
- NIH [2U01A1045452]
- USA NIH grant [AI054461]
- Department of Microbiology, Immunology and Pathology, CSU
We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species.
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